7LNH

S-adenosylmethionine synthetase co-crystallized with UppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 63 2 2
Unit cell lengths	144.191, 144.191, 188.196
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.400 - 2.500
R-factor	0.2208
Rwork	0.219
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a1g
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.400	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	40572	4502
<I/σ(I)>	17.3
Completeness [%]	99.9
Redundancy	39
CC(1/2)	0.999	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	0.1 M Bis-Tris pH 6.5 10% ethylene glycol PEG3350 10%