7LMT
Histone-lysine N-methyltransferase NSD2-PWWP1 with compound MRT10241866a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 1 |
Unit cell lengths | 68.300, 69.100, 80.192 |
Unit cell angles | 75.52, 79.36, 60.47 |
Refinement procedure
Resolution | 43.250 - 2.270 |
R-factor | 0.221 |
Rwork | 0.218 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xcg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.3) |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.810 | 48.810 | 2.330 |
High resolution limit [Å] | 2.260 | 9.330 | 2.260 |
Rmerge | 0.062 | 0.018 | 0.982 |
Rmeas | 0.084 | 0.025 | 1.338 |
Rpim | 0.056 | 0.017 | 0.902 |
Total number of observations | 108433 | 1526 | 8392 |
Number of reflections | 51608 | 741 | 4078 |
<I/σ(I)> | 8.6 | 33.7 | 0.9 |
Completeness [%] | 89.9 | 89.8 | 82 |
Redundancy | 2.1 | 2.1 | 2.1 |
CC(1/2) | 0.998 | 0.999 | 0.508 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 25% PEG3350, 0.2 M magnesium chloride, 0.1M HEPES, pH 7.5 |