7LMR
Structure of full-length human lambda-6A light chain JTO in complex with stabilizer 63 [4-methyl-3-(morpholinomethyl)-7-(2-phenylpropoxy)-2H-chromen-2-one]
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.742, 81.938, 95.826 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.960 - 2.090 |
R-factor | 0.2046 |
Rwork | 0.202 |
R-free | 0.26060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mg5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.584 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.960 | 2.200 |
High resolution limit [Å] | 2.090 | 2.090 |
Rpim | 0.034 | 0.251 |
Number of reflections | 30392 | 4309 |
<I/σ(I)> | 12.3 | 2.9 |
Completeness [%] | 99.7 | 98.1 |
Redundancy | 7.2 | 7.6 |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 20% PEG 3350 and 0.25 M NH4H2PO4 at 23 degrees C |