7LMQ

Structure of full-length human lambda-6A light chain JTO in complex with stabilizer 62 [4-methyl-3-(morpholinomethyl)-7-(1-phenylethoxy)-2H-chromen-2-one]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976484
Spacegroup name	P 21 21 21
Unit cell lengths	64.133, 81.090, 96.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.630 - 1.910
R-factor	0.1627
Rwork	0.161
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mg5
RMSD bond length	0.012
RMSD bond angle	1.724
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.630	2.010
High resolution limit [Å]	1.910	1.910
Rpim	0.037	0.278
Number of reflections	39458	5381
<I/σ(I)>	12.6	2.3
Completeness [%]	99.1
Redundancy	7.7
CC(1/2)	1.000	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	20% PEG 3350 and 0.25 M NH4H2PO4 at 23 degrees C