7LLI
Stimulatory immune receptor protein complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | H 3 |
| Unit cell lengths | 296.976, 296.976, 122.635 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 88.750 - 3.200 |
| R-factor | 0.2528 |
| Rwork | 0.244 |
| R-free | 0.26290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gup 1tvd 4lfh 5d5m |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.609 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.750 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 66481 | 4465 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 8.4 | |
| CC(1/2) | 0.990 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30-40% PEG 400, 0.1/0.2 HEPES pH 7.5, 0.2M MgCl2 |
