7LLA
Structure of human ATP citrate lyase in complex with acetyl-CoA and oxaloacetate (EM map was generated in Cryosparc with non-uniform refinement)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.97 |
| Number of Particle | 719613 |
| Software | RELION,SerialEM,Gctf,UCSF Chimera,PHENIX |
| Symmetry | D2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
