7LL8

D-Protein RFX-V1 Bound to the VEGFR1 Domain 2 Site on VEGF-A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	61.867, 69.080, 89.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.130 - 2.310
R-factor	0.263
Rwork	0.260
R-free	0.31420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qtk
RMSD bond length	0.005
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.130	46.090	2.390
High resolution limit [Å]	2.310	8.950	2.310
Rmerge	0.051	0.024	0.267
Rmeas	0.056	0.027	0.290
Rpim	0.023	0.012	0.113
Total number of observations		1842	10912
Number of reflections	17391	355	1680
<I/σ(I)>	25.9	51.8	7
Completeness [%]	99.9	99.2	100
Redundancy	6.4	5.2	6.5
CC(1/2)	0.996	0.999	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.2	calcium chloride, Tris, PEG4000