7LKI
The crystal structure of Epitope III of HCV envelop protein E2 in complex with antibody 1H8
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 201.683, 42.793, 110.824 |
Unit cell angles | 90.00, 109.53, 90.00 |
Refinement procedure
Resolution | 35.699 - 2.000 |
Rwork | 0.245 |
R-free | 0.29440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zea |
RMSD bond length | 0.006 |
RMSD bond angle | 1.513 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
Rmerge | 0.091 | 0.050 | 0.511 |
Rmeas | 0.110 | 0.059 | 0.660 |
Rpim | 0.060 | 0.031 | 0.412 |
Total number of observations | 212753 | ||
Number of reflections | 73746 | 4047 | 2699 |
<I/σ(I)> | 8.1 | ||
Completeness [%] | 95.3 | 99.2 | 70.2 |
Redundancy | 2.9 | 3.5 | 2 |
CC(1/2) | 0.995 | 0.476 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 20% PEG3350 0.1 M citrate pH 5.5 |