7LKA
Crystal structure of SARS-CoV-2 RBD-targeting antibody COV107-23
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 79.421, 74.797, 172.676 |
Unit cell angles | 90.00, 99.41, 90.00 |
Refinement procedure
Resolution | 31.005 - 1.981 |
R-factor | 0.2176 |
Rwork | 0.215 |
R-free | 0.26370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xc2 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
Rmerge | 0.140 | 0.052 | 1.468 |
Rmeas | 0.151 | 0.057 | 1.608 |
Rpim | 0.057 | 0.021 | 0.643 |
Total number of observations | 882641 | ||
Number of reflections | 130797 | 6520 | 6496 |
<I/σ(I)> | 4.4 | ||
Completeness [%] | 94.3 | 91 | 94.5 |
Redundancy | 6.7 | 6.9 | 5.5 |
CC(1/2) | 0.998 | 0.663 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 0.085 M of sodium citrate - citric acid pH 5.6, 0.17 M ammonium acetate, 15% (v/v) glycerol, 25.5% (w/v) polyethylene glycol 4000 |