7LK3
Crystal structure of untwinned human GABARAPL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.402, 58.700, 67.997 |
| Unit cell angles | 90.00, 98.27, 90.00 |
Refinement procedure
| Resolution | 33.650 - 1.900 |
| Rwork | 0.196 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4co7 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.650 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.929 | 0.918 |
| Rmeas | 0.988 | |
| Number of reflections | 17461 | 1693 |
| <I/σ(I)> | 14.55 | 2 |
| Completeness [%] | 99.6 | 99.94 |
| Redundancy | 7 | 7.2 |
| CC(1/2) | 0.999 | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.15 M KBr, 30% (w/v) PEG monomethyl ether 2000 |
