7LJB

Human TRAAK K+ channel mutant G158D in a K+ bound conductive conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2019-06-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	80.140, 137.310, 96.300
Unit cell angles	90.00, 94.72, 90.00

Refinement procedure

Resolution	48.030 - 2.970
R-factor	0.1981
Rwork	0.196
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wff
RMSD bond length	0.009
RMSD bond angle	1.579
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	3.232
High resolution limit [Å]	2.970	2.970
Rmerge	0.207	2.023
Rmeas		2.109
Rpim		0.597
Number of reflections	31745	1984
<I/σ(I)>	8.5	1.234
Completeness [%]	93.6	21.23
Redundancy	11.6	6.22
CC(1/2)	0.999	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	277	50mM Tris pH 8.8, 64-200 mM CaCl2, 27-33%(vol/vol) PEG400.