7LJ5

Human TRAAK K+ channel FHIEG mutant A198E in a K+ bound conductive conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2019-10-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.11583
Spacegroup name	P 1 21 1
Unit cell lengths	80.160, 136.980, 95.740
Unit cell angles	90.00, 94.47, 90.00

Refinement procedure

Resolution	95.450 - 2.260
R-factor	0.2216
Rwork	0.220
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wfe
RMSD bond length	0.007
RMSD bond angle	1.406
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.450	2.504
High resolution limit [Å]	2.260	2.260
Rmerge		2.827
Rmeas		2.884
Rpim		0.567
Number of reflections	57752	2875
<I/σ(I)>	10.2	1.508
Completeness [%]	93.8	12
Redundancy	27.2	12.88
CC(1/2)	0.971	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	277	50mM Tris pH 8.8, 64-200 mM CaCl2, 27-33 vol vol PEG400