7LH9
Crystal structure of BRPF2 PWWP domain in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.410, 216.113, 54.037 |
| Unit cell angles | 90.00, 109.42, 90.00 |
Refinement procedure
| Resolution | 41.640 - 2.600 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.27840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z02 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.355 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.094 | 0.051 | 0.862 |
| Rmeas | 0.110 | 0.059 | 1.019 |
| Rpim | 0.057 | 0.029 | 0.534 |
| Total number of observations | 92761 | ||
| Number of reflections | 25975 | 1354 | 1339 |
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 97.1 | 98.9 | 98.5 |
| Redundancy | 3.6 | 3.8 | 3.5 |
| CC(1/2) | 0.997 | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG 3350, 0.2M Magnesium Acetate |
