7LFC
Structure of importin a3 bound to p50 NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.953 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.650, 59.070, 86.090 |
| Unit cell angles | 90.00, 96.34, 90.00 |
Refinement procedure
| Resolution | 14.930 - 2.100 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tbk |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.044 | 0.412 |
| Number of reflections | 27642 | 2009 |
| <I/σ(I)> | 11.6 | 2.4 |
| Completeness [%] | 98.8 | 87.7 |
| Redundancy | 5.5 | |
| CC(1/2) | 0.998 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2M potassium thiocyanate, 20% PEG3350, 0.1 M BIS-TRIS pH 6.5 |
