7LF7
Fab 6D12 bound to ApoL1 NTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2016-11-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 |
Spacegroup name | P 43 |
Unit cell lengths | 131.103, 131.103, 87.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.458 - 2.026 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fvd |
RMSD bond length | 0.003 |
RMSD bond angle | 0.771 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.704 | 2.152 |
High resolution limit [Å] | 2.026 | 2.027 |
Rmerge | 0.073 | 0.956 |
Rmeas | 0.083 | 1.250 |
Rpim | 0.051 | 0.797 |
Number of reflections | 76896 | 3846 |
<I/σ(I)> | 12.8 | 1.5 |
Completeness [%] | 95.1 | 64.4 |
Redundancy | 4.5 | 4.5 |
CC(1/2) | 0.998 | 0.479 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 20% PEG 3350, 0.1M Ammonium Sulfate, 0.1M MES pH 6, 14mM sodium cholate |