7LF5
Structure of Hyperglycosylated Human IgG1 Fc (Fc267)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.033200 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.379, 146.831, 75.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.390 - 2.600 |
R-factor | 0.2247 |
Rwork | 0.224 |
R-free | 0.24270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cdh |
RMSD bond length | 0.010 |
RMSD bond angle | 1.331 |
Data reduction software | XDS (1.18.2_3874) |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.390 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.021 | |
Rpim | 0.022 | 0.387 |
Number of reflections | 9106 | 1110 |
<I/σ(I)> | 13.9 | 2 |
Completeness [%] | 99.4 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | CRYSTALS GROWN BY MIXING 1 UL OF FC267 (10 MG/ML IN 10mM HEPES, 75mM NaCl pH 7.4) WITH 1 UL OF PRECIPITANT SOLUTION CONSISTING OF 0.1M HEPES pH 6.5, 8% w/v PEG6000 |