7LBQ
Crystal structure of human Survivin bound to histone H3 T3phK4me2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97926 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 69.343, 70.162, 89.447 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.360 - 2.690 |
R-factor | 0.2494 |
Rwork | 0.248 |
R-free | 0.26840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uec |
RMSD bond length | 0.017 |
RMSD bond angle | 2.063 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.075 | 0.059 | 0.758 |
Total number of observations | 43026 | ||
Number of reflections | 6766 | 325 | 356 |
<I/σ(I)> | 18.2 | ||
Completeness [%] | 99.1 | 84.4 | 100 |
Redundancy | 6.4 | 5.1 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 1 uL of protein was mixed with 1uL of buffer composed of 2.25 mM spermine, 9 mM MgCl2, 0.9 mM spermidine, 1.8 mM cobalt (III)hexamine chloride, 0.05 sodium cacodylate pH 7.0, 5% PEG 400 |