7LBL

Structure of Human IgG1 Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.033200
Spacegroup name	P 21 21 21
Unit cell lengths	49.523, 79.986, 141.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.840 - 2.130
R-factor	0.2151
Rwork	0.214
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jii
RMSD bond length	0.011
RMSD bond angle	1.310
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.840	28.840	2.190
High resolution limit [Å]	2.130	9.040	2.130
Rmerge	0.048	0.027	0.765
Rpim	0.021	0.012	0.326
Number of reflections	32029	492	2568
<I/σ(I)>	2.28
Completeness [%]	99.1		98.5
Redundancy	5.9		6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	CRYSTALS GROWN BY MIXING 1 UL OF FC (10 MG/ML IN 10mM HEPES, 75mM NaCl pH 7.4) WITH 1 UL OF PRECIPITANT SOLUTION CONSISTING OF 0.05M Phosphate PH7, 20% PEG 3350 pH 7.4