7LA5

Structure of human GGT1 in complex with Lnt1-172 compound.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	103.223, 118.855, 101.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.930 - 2.070
R-factor	0.2252
Rwork	0.223
R-free	0.28090
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4z9o
RMSD bond length	0.010
RMSD bond angle	1.489
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.8.0073)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.130
High resolution limit [Å]	2.070	2.070
Rmerge	0.129	0.781
Rmeas	0.142	0.876
Rpim	0.058	0.383
Number of reflections	36505	2507
<I/σ(I)>	11.25	1.88
Completeness [%]	96.9	94.05
Redundancy	5.4	4.1
CC(1/2)		0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	20-25%PEG3350, 0.1M ammonium cloride, 0.1M sodium cacodilate pH 6.0