7L9E

Crystal structure of apo-alpha glucosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.997
Spacegroup name	P 32
Unit cell lengths	102.966, 102.966, 240.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.330 - 2.290
R-factor	0.18
Rwork	0.180
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f0e
Data reduction software	HKL-2000 (v717.1)
Data scaling software	HKL-2000 (v717.1)
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.330	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.125
Number of reflections	128793	12699
<I/σ(I)>	8.246
Completeness [%]	99.8	99.73
Redundancy	2.8	2.7
CC(1/2)	0.967	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.09 M NPS, 0.1 M Buffer System 1 (pH 7.0), 29% (v/v) P500MME P20K