7L97

Crystal structure of STAMBPL1 in complex with an engineered binder

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	63.709, 68.882, 57.329
Unit cell angles	90.00, 98.51, 90.00

Refinement procedure

Resolution	50.000 - 2.010
R-factor	0.1859
Rwork	0.185
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2znr 1ubq
RMSD bond length	0.010
RMSD bond angle	1.377
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.123	0.075	0.700
Rmeas	0.139	0.084	0.814
Rpim	0.063	0.039	0.405
Number of reflections	16078	845	786
<I/σ(I)>	7.2
Completeness [%]	99.0	99.8	96.4
Redundancy	4.4	4.5	3.4
CC(1/2)		0.991	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	20% PEG 1500, 0.2M NaCl, 0.1M HEPES pH7.5