7L97
Crystal structure of STAMBPL1 in complex with an engineered binder
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 63.709, 68.882, 57.329 |
Unit cell angles | 90.00, 98.51, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.010 |
R-factor | 0.1859 |
Rwork | 0.185 |
R-free | 0.21370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2znr 1ubq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.377 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.6.1) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.123 | 0.075 | 0.700 |
Rmeas | 0.139 | 0.084 | 0.814 |
Rpim | 0.063 | 0.039 | 0.405 |
Number of reflections | 16078 | 845 | 786 |
<I/σ(I)> | 7.2 | ||
Completeness [%] | 99.0 | 99.8 | 96.4 |
Redundancy | 4.4 | 4.5 | 3.4 |
CC(1/2) | 0.991 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20% PEG 1500, 0.2M NaCl, 0.1M HEPES pH7.5 |