7L8R
Crystal structure of human GPX4-U46C mutant K48A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-29 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.804, 68.559, 81.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.480 - 1.520 |
R-factor | 0.1595 |
Rwork | 0.158 |
R-free | 0.17720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7l8k |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.550 | 1.540 |
High resolution limit [Å] | 1.520 | 1.520 |
Number of reflections | 54881 | 1615 |
<I/σ(I)> | 13.2 | |
Completeness [%] | 100.0 | |
Redundancy | 12.9 | |
CC(1/2) | 0.990 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6 | 293 | 0.1 M sodium chloride, 0.1 M MES, and 40% (w/v) PEG 8000 |