7L8Q
Crystal structure of human GPX4-U46C with oxidized Cys-46
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.757, 69.035, 35.678 |
| Unit cell angles | 90.00, 115.88, 90.00 |
Refinement procedure
| Resolution | 34.520 - 1.480 |
| R-factor | 0.157 |
| Rwork | 0.154 |
| R-free | 0.18210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7l8k |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.040 | 1.500 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 23698 | 1049 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 99.0 | 87.4 |
| Redundancy | 3.3 | 2.8 |
| CC(1/2) | 0.990 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 293 | 0.1 M potassium thiocyanate, 0.1 M sodium acetate, and 20% (w/v) PEG 8000 |
