7L7X
X-ray structure of the Pcryo_0638 aminotransferase from Psychrobacter cryohalolentis
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-07 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.529, 63.141, 69.853 |
| Unit cell angles | 81.75, 78.59, 66.82 |
Refinement procedure
| Resolution | 29.957 - 1.300 |
| Rwork | 0.150 |
| R-free | 0.16920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3frk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.472 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 202026 | 36011 |
| <I/σ(I)> | 7.2 | 1.9 |
| Completeness [%] | 92.9 | 83 |
| Redundancy | 2.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 18-22% PEG-8000, 200 mM tetramethylammonium chloride. protein incubated with 1 mM PLP and 5 mM UDP-2-acetamido-4-amino-2,4,6-trideoxy-D-glucose |
