7L3L

Structure of TRAF5 and TRAF6 RING Hetero dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-08
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.953700
Spacegroup name	P 1 21 1
Unit cell lengths	41.597, 106.062, 94.427
Unit cell angles	90.00, 102.64, 90.00

Refinement procedure

Resolution	42.250 - 2.800
R-factor	0.2102
Rwork	0.204
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vo0
RMSD bond length	0.004
RMSD bond angle	0.798
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.254	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.050	0.440
Rpim	0.050	0.440
Number of reflections	19772	1970
<I/σ(I)>	13.49	1.81
Completeness [%]	99.9	100
Redundancy	2
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8	291	0.1 M Tris (pH 8), 0.2 M NaCl, 20 % PEG 6000