7L1G
PRMT5-MEP50 Complexed with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97890 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 101.548, 138.780, 178.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.070 - 2.470 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6uxx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.750 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.460 | 2.470 |
| Rmerge | 0.100 | 0.928 |
| Number of reflections | 44543 | 3694 |
| <I/σ(I)> | 29.3 | 2.7 |
| Completeness [%] | 97.3 | 81.9 |
| Redundancy | 12.9 | 11.9 |
| CC(1/2) | 0.998 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M sodium citrate pH 6.0, 0.2 M sodium acetate, 10-12% PEG 4000 |
