7L18
Crystal structure of a tandem deletion mutant of rat NADPH-cytochrome P450 reductase
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 1999-11-22 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.058, 114.821, 120.285 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.787 - 2.542 |
Rwork | 0.181 |
R-free | 0.25380 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1amo |
RMSD bond length | 0.006 |
RMSD bond angle | 1.415 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.8.0267) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.263 | |
Number of reflections | 43215 | 1879 |
<I/σ(I)> | 16.7 | |
Completeness [%] | 91.9 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 18% PEG4000, 150 mM HEPES, pH 7.0, 150 mM sodium acetate, protein, two-fold molar excess of NADP+ |