7L0F

Monobody 12VC3 Bound to HRAS(WT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	73.012, 64.816, 127.124
Unit cell angles	90.00, 102.37, 90.00

Refinement procedure

Resolution	44.880 - 1.980
R-factor	0.161
Rwork	0.159
R-free	0.19120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g0n
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (v5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.980	5.370	1.980
Rmerge	0.115	0.070	0.767
Rmeas	0.128	0.078	0.854
Rpim	0.056	0.034	0.370
Total number of observations	388685
Number of reflections	77326	3965	3839
<I/σ(I)>	7.9
Completeness [%]	96.0	94.9	95.3
Redundancy	5	4.9	4.9
CC(1/2)		0.995	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350