7KX6

Crystal structure of DCLK1-KD in complex with XMD8-85

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	I 1 2 1
Unit cell lengths	66.180, 63.330, 152.491
Unit cell angles	90.00, 100.47, 90.00

Refinement procedure

Resolution	56.093 - 2.500
R-factor	0.1941
Rwork	0.192
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jzj
RMSD bond length	0.004
RMSD bond angle	0.707
Data scaling software	Aimless (0.5.8)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.980	74.980	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.117	0.038	0.674
Rmeas	0.132	0.043	0.760
Rpim	0.060	0.019	0.345
Total number of observations	101629	2314	11767
Number of reflections	21500	497	2436
<I/σ(I)>	10.8	25.1	2.9
Completeness [%]	99.1	99.7	99.7
Redundancy	4.7	4.7	4.8
CC(1/2)	0.993	0.999	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.25	298	PEG400, ammonium sulfphate, Hepes