7KX4

Anti-CCHFV ADI-36121 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	58.190, 76.640, 208.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.490 - 2.600
R-factor	0.2
Rwork	0.198
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i19 1hez
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.500	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	29766	29695
<I/σ(I)>	8.1
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.987	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MgCl2, 0.1 M CaCl2, 0.1 M KNa Tartrate, 25% PEG 4000, 4% isopropanol, 0.1 M Bis-Tris-HCl pH 6.5