7KUF

Transcription activation subcomplex with WhiB7 bound to SigmaAr4-RNAP Beta flap tip chimera and DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	69.254, 69.254, 167.374
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.990 - 2.600
R-factor	0.2227
Rwork	0.221
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kug
RMSD bond length	0.007
RMSD bond angle	0.945
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.101
Number of reflections	14675	612
<I/σ(I)>	33.1
Completeness [%]	98.1	83.3
Redundancy	26.4
CC(1/2)	0.994	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.2-0.3 M NH4H2PO4