7KTS
Negative stain EM structure of the human SAGA coactivator complex (TRRAP, core, splicing module)
This is a non-PDB format compatible entry.
EM specimen
| Staining | Yes |
| Staining material | uranyl formate |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2.2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 19.09 |
| Number of Particle | 45790 |
| Software | RELION,PHENIX,Leginon,CTFFIND,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
