7KTE
DNA Polymerase Mu, 8-oxodGTP:Ct Reaction State Ternary Complex, 50 mM Mg2+ (90min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.940, 68.523, 110.605 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.958 - 1.479 |
R-factor | 0.1578 |
Rwork | 0.157 |
R-free | 0.17740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.510 |
High resolution limit [Å] | 1.479 | 4.020 | 1.480 |
Rmerge | 0.136 | 0.122 | 0.886 |
Rmeas | 0.149 | 0.134 | 0.974 |
Rpim | 0.060 | 0.056 | 0.397 |
Number of reflections | 76692 | 4107 | 3758 |
<I/σ(I)> | 11.1 | ||
Completeness [%] | 99.9 | 99.4 | 99.9 |
Redundancy | 6.2 | 5.8 | 5.9 |
CC(1/2) | 0.973 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |