7KT9

DNA Polymerase Mu, 8-oxodGTP:At Product State Ternary Complex, 50 mM Mg2+ (960min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.283, 68.316, 111.407
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.201 - 1.482
R-factor	0.1562
Rwork	0.155
R-free	0.17550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.510
High resolution limit [Å]	1.480	4.020	1.480
Rmerge	0.125	0.113	0.571
Rmeas	0.138	0.125	0.626
Rpim	0.056	0.052	0.252
Total number of observations	444209
Number of reflections	76825	4066	3800
<I/σ(I)>	12
Completeness [%]	99.7	98.3	99.9
Redundancy	5.8	5.4	5.9
CC(1/2)		0.978	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000