7KSU
DNA Polymerase Mu, dGTP:Ct Product State Ternary Complex, 10 mM Mn2+ (4min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-08-17 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.985, 68.882, 109.747 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.823 - 1.650 |
R-factor | 0.174 |
Rwork | 0.173 |
R-free | 0.19160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.953 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 4.480 | 1.650 |
Rmerge | 0.143 | 0.156 | 0.976 |
Rmeas | 0.156 | 0.170 | 1.062 |
Rpim | 0.060 | 0.066 | 0.410 |
Total number of observations | 370613 | ||
Number of reflections | 54904 | 2968 | 2696 |
<I/σ(I)> | 9.6 | ||
Completeness [%] | 99.4 | 98.9 | 99.5 |
Redundancy | 6.8 | 6.4 | 6.2 |
CC(1/2) | 0.968 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG 4000 |