7KQ7
Crystal structure of IL21R in complex with an antibody Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 230 |
Detector technology | PIXEL |
Collection date | 2010-05-05 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 155.482, 246.501, 55.063 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.216 - 2.203 |
R-factor | 0.2161 |
Rwork | 0.215 |
R-free | 0.24040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in house structure |
RMSD bond length | 0.009 |
RMSD bond angle | 1.191 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | 0.580 |
Number of reflections | 47436 | 1437 |
<I/σ(I)> | 18.8 | |
Completeness [%] | 87.8 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 10% PEG MME 5000 and 0.1M MES, pH 6.5 |