7KPU

Crystal structure of human NatD (NAA40) bound to a bisubstrate analogue with a C-3 linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	46.317, 74.164, 127.022
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.050 - 1.430
R-factor	0.1636
Rwork	0.163
R-free	0.18270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u9w
RMSD bond length	0.013
RMSD bond angle	1.489
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	127.020	1.450
High resolution limit [Å]	1.430	1.430
Rmerge	0.037	0.268
Rmeas	0.040	0.295
Rpim	0.016	0.120
Number of reflections	81356	3986
<I/σ(I)>	35.9	6.2
Completeness [%]	99.5
Redundancy	6.5
CC(1/2)	0.997	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1 M Bis-tris, pH 5.5, 2M Ammonium sulfate