7KPK
Crystal structure of the SPOP MATH domain in complex with a fragment of Pdx1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.7999898 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.796, 53.985, 41.556 |
| Unit cell angles | 90.00, 101.43, 90.00 |
Refinement procedure
| Resolution | 30.750 - 1.710 |
| R-factor | 0.1835 |
| Rwork | 0.181 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ivb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.350 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 13113 | 684 |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 89.7 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.982 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 50 mM sodium cacodylate, pH 6.5, 0.08 M magnesium acetate, 30% (w/v) PEG 4000 |
