7KPJ
Crystal structure of Ruminococcus gnavus immunoglobulin binding protein in complex with 338E6 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.240, 102.191, 118.280 |
Unit cell angles | 90.00, 102.05, 90.00 |
Refinement procedure
Resolution | 83.360 - 2.100 |
R-factor | 0.2299 |
Rwork | 0.229 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dee |
RMSD bond length | 0.008 |
RMSD bond angle | 0.992 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.360 | 2.177 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 115284 | 11391 |
<I/σ(I)> | 21.03 | |
Completeness [%] | 99.1 | 98.16 |
Redundancy | 33.4 | 32.5 |
CC(1/2) | 0.971 | 0.307 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.2 M calcium chloride, 18% PEG 4000 |