7KMQ

Crystal structure of the GH95 alpha-L-1,2-fucosidase (Xac1774) from Xanthomonas citri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.5400
Spacegroup name	P 21 21 21
Unit cell lengths	103.600, 103.520, 173.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.781 - 2.045
R-factor	0.1749
Rwork	0.174
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ufc
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3139)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.781	2.170
High resolution limit [Å]	2.045	2.045
Number of reflections	117432	17998
<I/σ(I)>	13.15	1.31
Completeness [%]	96.8	96.1
Redundancy	3.42	3.23
CC(1/2)	0.998	0.465

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1 mol/L tris 20% (v/v) 2-methyl-2,4-pentamidiol