7KMI
LY-CoV481 neutralizing antibody against SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.239, 74.046, 126.086 |
| Unit cell angles | 90.00, 111.85, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.730 |
| R-factor | 0.1875 |
| Rwork | 0.186 |
| R-free | 0.21720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7kmg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.244 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.033 | 0.418 |
| Number of reflections | 93105 | 13500 |
| <I/σ(I)> | 5.9 | 1.5 |
| Completeness [%] | 98.7 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.999 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 14% PEG 4000, 10% 2-Propanol, 100 mM Tri-Sodium Citrate pH 5.8 |
