7KMH
LY-CoV488 neutralizing antibody against SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.806, 260.590, 94.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.390 - 1.720 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7kmg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.820 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.051 | 0.630 |
| Number of reflections | 97212 | 14015 |
| <I/σ(I)> | 13.4 | 3.4 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 13 | 13.4 |
| CC(1/2) | 0.999 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293 | 8% PEG 3350, 200 mM L-Proline, 100 mM Hepes pH 7.7 |
