7KLZ
Structure of SPOP MATH domain in complex with a Geminin peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-10-14 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9786 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 103.060, 103.060, 131.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.990 - 3.400 |
R-factor | 0.213 |
Rwork | 0.210 |
R-free | 0.24140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f8g |
RMSD bond length | 0.002 |
RMSD bond angle | 0.479 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.990 | 3.520 |
High resolution limit [Å] | 3.390 | 3.390 |
Rmerge | 0.096 | 1.270 |
Number of reflections | 10264 | 985 |
<I/σ(I)> | 15.24 | |
Completeness [%] | 99.8 | |
Redundancy | 37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | One microliter of the protein complex in 20 mM Tris-HCl, pH 7.6, 50 mM NaCl and 5 mM dithiothreitol was mixed with on microliter of 0.2 M ammonium citrate dibasic and 20% (W/V) PEG 3350 |