7KLD
Crystal Structure of an Essential Ribosomal Processing Protease Prp from S. aureus in complex with a covalently linked product Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-26 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.54184 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 53.482, 66.556, 93.446 |
Unit cell angles | 90.00, 103.44, 90.00 |
Refinement procedure
Resolution | 29.310 - 2.250 |
R-factor | 0.2472 |
Rwork | 0.245 |
R-free | 0.27890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4peo |
RMSD bond length | 0.020 |
RMSD bond angle | 1.835 |
Data reduction software | CrysalisPro (40.64.42a) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.310 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.148 | 0.602 |
Rmeas | 0.155 | 0.632 |
Rpim | 0.047 | 0.190 |
Number of reflections | 15213 | 14933 |
<I/σ(I)> | 3.84 | 4.4 |
Completeness [%] | 95.2 | 100 |
Redundancy | 10.8 | 10.9 |
CC(1/2) | 0.997 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Protein sample: 10 - 20 mg/ml in 10mM Hepes (pH 7.5), 0.1M NaCl, 0.3mM TCEP, 3.3mM Triss (pH 7.5), 0.5% beta-octylglucoside, <4% glycerol |