7KL6
Helicobacter pylori Xanthine-Guanine-Hypoxanthine Phosphoribosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-10-12 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.3776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.221, 51.488, 82.879 |
| Unit cell angles | 90.00, 96.21, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.970 |
| R-factor | 0.162 |
| Rwork | 0.161 |
| R-free | 0.19260 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.202 |
| Data reduction software | ARP/wARP |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | Auto-Rickshaw (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.780 | 2.080 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 24376 | 2370 |
| <I/σ(I)> | 74.96 | |
| Completeness [%] | 99.5 | |
| Redundancy | 14 | |
| CC(1/2) | 0.800 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 273 | 0.1 M Bis-Tris propane, pH 5.5, 0.2 M ammonium sulfate, 25 % PEG3350 |
