7KL5
Structure of Calmodulin Bound to the Cardiac Ryanodine Receptor (RyR2) at Residues: Phe4246 to Val4271
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.169, 62.403, 43.190 |
Unit cell angles | 90.00, 107.49, 90.00 |
Refinement procedure
Resolution | 41.192 - 1.650 |
Rwork | 0.209 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bcx |
RMSD bond length | 0.015 |
RMSD bond angle | 1.804 |
Data reduction software | XDS (VERSION Jan 26, 2018 BUILT=20180319) |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.400 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.050 | 0.865 |
Rmeas | 0.060 | 1.030 |
Rpim | 0.032 | |
Number of reflections | 19805 | 3226 |
<I/σ(I)> | 9.3 | 1.5 |
Completeness [%] | 97.9 | 96.7 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.997 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2M sodium acetate, 0.1M Tris pH 8.5, 30%(w/v) PEG 4000 |