7KKM
Structure of the catalytic domain of tankyrase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2018-11-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.030, 114.930, 80.520 |
Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
Resolution | 22.820 - 1.580 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rf5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.500 | 1.710 |
High resolution limit [Å] | 1.580 | 1.580 |
Number of reflections | 92574 | 4579 |
<I/σ(I)> | 14.8 | |
Completeness [%] | 89.2 | |
Redundancy | 3.2 | |
CC(1/2) | 0.999 | 0.611 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | Precipitant: 20.0 %v/v Ethylene glycol Precipitant: 10.0 %w/v PEG 4000 Salt: 0.1 M Sodium chloride Buffer: 0.1 M MES (pH 6.00) |