7KKF
Crystal Structure of S. cerevisiae Ess1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.917 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.098, 57.366, 69.334 |
Unit cell angles | 90.00, 96.92, 90.00 |
Refinement procedure
Resolution | 29.210 - 2.400 |
R-factor | 0.2712 |
Rwork | 0.269 |
R-free | 0.30650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yw5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.006 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.392 | 5.170 | 2.400 |
Rmerge | 0.099 | 0.070 | 0.712 |
Rmeas | 0.107 | 0.076 | 0.768 |
Rpim | 0.040 | 0.030 | 0.286 |
Number of reflections | 16821 | 1721 | 1694 |
<I/σ(I)> | 10.8 | ||
Completeness [%] | 99.8 | 98.8 | 100 |
Redundancy | 7 | 6.6 | 7 |
CC(1/2) | 0.996 | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris, pH 7.7 and 21% (w/v) PEG 8000 |