7KJH

Plasmodium falciparum protein Pf12p bound to nanobody B9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953725
Spacegroup name	P 21 21 21
Unit cell lengths	84.986, 107.044, 113.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.338 - 2.000
R-factor	0.1814
Rwork	0.180
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ymo 6waq
RMSD bond length	0.008
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.338	47.338	2.120
High resolution limit [Å]	2.000	5.960	2.000
Rmerge	0.195	0.070	1.434
Rmeas	0.203	0.073	1.487
Total number of observations	978968
Number of reflections	70873	2878	11300
<I/σ(I)>	10.73	33.22	1.6
Completeness [%]	100.0	99.7	99.9
Redundancy	13.813	12.54	14.126
CC(1/2)	0.998	0.999	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	281	20% PEG3000, 0.1 M trisodium citrate-citric acid pH 5.5