7KJB

Crystal structure of the EphA2 S897E/S901E mutant intracellular KD-SAM domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-04-07
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	94.456, 94.456, 99.646
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.530 - 2.800
R-factor	0.2
Rwork	0.198
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pdo 3kka
RMSD bond length	0.003
RMSD bond angle	0.499
Data reduction software	XDS (VERSION Oct 15, 2015)
Data scaling software	Aimless (version 0.5.23)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.530	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.285	1.956
Rmeas	0.313	2.153
Rpim	0.130	0.895
Number of reflections	13051	1871
<I/σ(I)>	10.2	1.4
Completeness [%]	99.9	100
Redundancy	10.9	11
CC(1/2)	0.992	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	287	4.5% Tacsimate, 0.09 M HEPES pH 7.0, 9% PEG-MME 5K, 0.1 M CsCl